1. Nanoscale
visualization, Nanoparticle bio-labels, Nanotechnology-based
biochips/microarrays, Nanoparticle-based nucleic acid diagnostics and Bio-barcode
assays
4. DNA-Based/Genetic monitoring; Immunosensors/HIV,
hepatitis and other viral diseases, drug testing; Cell-based/Drug testing; Point-of-care
Sensors/Blood and urine testing; Bacterial Sensors/ Bacterial infection identification
7. The advantages of cantilevers are that they
provide fast, label-free detection of biomolecules.
10. It is an integrated proteomics approach using
chemically functionalized gold nanoparticles (AuNP) as probes for affinity
purification of large protein complexes.
These complexes were dissected using standard mass spectroscopy (MS)
techniques to comprehensively identify individual proteins present in a
sample. In their approach, nucleotide sequences
that specifically bind protein complexes in the nucleus of cells were linked to
the AuNPs and were functionalized with PEG.
Functionalized AuNPs were incubated with whole cell lysate extracts
(extracts of protein/DNA complexes from the entire cell, nucleus and cytoplasm)
of the cell line MCF-7 and characterized for the presence of protein binding
complexes by MS. See Figure 8.14 for a
schematic diagram of the system.
13. It is well known that gold induces strong X-ray
attenuation. In addition, gold
nanoparticles have unique physical properties that may make them ideal as in
vivo contrast agents, most importantly including their flexibility with
respect to functional group modification for the efficient attachment of
targeting species. The fact that gold
nanoparticles are generally accepted as non-toxic is also an important
consideration when designing nanoparticle-based contrast agents.
16. They addressed it by using an innovative
approach to develop magnetism-engineered iron oxide (MEIO) nanoparticles which
are novel metal-doped nanoparticles engineered to possess exceptionally high
and tunable nanomagnetism. The
nanoparticles were synthesized under high temperature in an organic medium for
control of purity, size uniformity, crystallinity, stoichiometry and
magnetism.
19. [NP] = Vnp/(Vnp*Ve*Nav)
22. See Figure 8.27 for a diagrammatic
illustration.
