Understanding Nanomedicine:  An Introductory Textbook
By Rob Burgess, PhD
Copyright 2011. Pan Stanford Publishing.  All Rights Reserved.

Chapter 8 Review Question Answers

Following are selected answers to the review questions at the end of Chapter 8 in Understanding Nanomedicine:  An Introductory Textbook by Rob Burgess.

1. Nanoscale visualization, Nanoparticle bio-labels, Nanotechnology-based biochips/microarrays, Nanoparticle-based nucleic acid diagnostics and Bio-barcode assays



4. DNA-Based/Genetic monitoring; Immunosensors/HIV, hepatitis and other viral diseases, drug testing; Cell-based/Drug testing; Point-of-care Sensors/Blood and urine testing; Bacterial Sensors/ Bacterial infection identification


7. The advantages of cantilevers are that they provide fast, label-free detection of biomolecules.



10. It is an integrated proteomics approach using chemically functionalized gold nanoparticles (AuNP) as probes for affinity purification of large protein complexes. These complexes were dissected using standard mass spectroscopy (MS) techniques to comprehensively identify individual proteins present in a sample. In their approach, nucleotide sequences that specifically bind protein complexes in the nucleus of cells were linked to the AuNPs and were functionalized with PEG. Functionalized AuNPs were incubated with whole cell lysate extracts (extracts of protein/DNA complexes from the entire cell, nucleus and cytoplasm) of the cell line MCF-7 and characterized for the presence of protein binding complexes by MS. See Figure 8.14 for a schematic diagram of the system.


13. It is well known that gold induces strong X-ray attenuation. In addition, gold nanoparticles have unique physical properties that may make them ideal as in vivo contrast agents, most importantly including their flexibility with respect to functional group modification for the efficient attachment of targeting species. The fact that gold nanoparticles are generally accepted as non-toxic is also an important consideration when designing nanoparticle-based contrast agents.



16. They addressed it by using an innovative approach to develop magnetism-engineered iron oxide (MEIO) nanoparticles which are novel metal-doped nanoparticles engineered to possess exceptionally high and tunable nanomagnetism. The nanoparticles were synthesized under high temperature in an organic medium for control of purity, size uniformity, crystallinity, stoichiometry and magnetism.


19. [NP] = Vnp/(Vnp*Ve*Nav)



22. See Figure 8.27 for a diagrammatic illustration.